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پاورپوینت The Immunoassay

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پاورپوینت The Immunoassay

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The Immunoassay

Chad Chisolm , Jaclyn Holcombe, Matthew Shelnutt

Know that Toxicology is the branch of Forensic Science in which the immunoassay plays the greatest role.

Know that in the year 1959, the RIA, predecessor of modern immunoassays, was invented.

Know that the RIA was invented by Drs. Yalow and Berson .

Know that antibodies are proteins that are found in blood or other bodily fluids that are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses .

Know that an antigen is any substance that causes your immune system to produce antibodies against it. An antigen may be a foreign substance from the environment such as chemicals, bacteria, viruses, or pollen; they may also be produced inside the body.

Objectives

Objectives Cont’d

Know that a hybridoma is the hybrid of an antibody and a myleoma cell, which are cultured to produce antibodies.

Know that immunoassays are typically categorized as either competitive or noncompetitive.

Know that in noncompetitive immunoassays, the amount of labeled analyte is directly proportional to the amount of antigen; in the competitive, it is inversely proportional.

Know that heterogeneous immunoassays depend on the separation of unbound tracers before the bound signal is measured; homogeneous immunoassays do not require this separation because the signal is generated when the binding occurs.

Know that two specific types of immunoassays include the EMIT, RIA, ELISA, FPIA.

History and Background

In the year 1959, Drs. Rosalyn Yalow & Soloman Berson invented the radioimmunoassay, which applied the use of

radioisotopes in the

measurement of

insulin.

The RIA is the predecessor of modern immunoassays.

Dr. Rosalyn Yalow became the first

female to win a Nobel Prize with

her work on the radioimmunoassay.

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